HPLC Chromatography: Understanding HPLC, Test Methods, Instrumentation & Columns

Cyano Column Zodiac Cyano column offers best peak shape and efficiency and with unique selectivity compared to C18 & C8 stationary phases. Zodiac Cyano, Cyanopropryl groups bonded to Silica gel, offers unique selectivity, very low operating back pressure, long column operating life and excellent peak shapes in both reverse phase and normal phase modes. Ready for LC/MS Applications. Cyano column TECHNICAL DETAILS Silica type           :  Spherical Silica Purity       :  99.999% Carbon Load     :  15% Surface Area      :  450 m2/gram Pore Size            :  100A pH Range           :  2.0-7.5 Particle Size       :  3µm,3.5µm, 5µm,10µm

RP Amide Column The Zodiac 120 RP Amide column is a reversed-phase HPLC column with an amide-based stationary phase, ideal for separating both polar and non-polar analytes. It offers high resolution and reproducibility in aqueous-organic mobile phases for various analytical applications. Separation of 1-methyl-4-ethylbenzene using RP Amide Column RP Amide Column Application Name  :  Reference  Reformate  Standard Benzoic acid Related Compounds Seperation using Zodiac 120 RP Amide Tenofovir Disoproxil Fumarate Analysis using Zodiac 120 RP-Amide Column Column Name  :  GRS – 1 GC Capillary Column Analytes  :  Benzene ,  Toluene ,  Naphthalene  ,  Ethylbenzene ,  Propylbenzene , Butylbenzene ,  Propane  ,   2-methylpentane ,  2,4-dimethylpentane ,  o-xylene ,  Isopentane ,  1,2,4-trimethylbenzene ,   2,3-dimethylbutane ,  Methylcyclopentane ,  m-xylene ,  p-xylen...

Amino Column Zodiac Amino Column has Aminopropyl groups bonded to Silica gel. Generally Amino columns are widely used for analysing sugar in reversed phase mode The Zodiac Amino Column can be utilized for normal-phase and weak anion-exchange compounds and can separate vitamins, carbohydrates and sugars in the reversed-phase mode. Amino Column Technical Details of Amino Column   Silica type  : Spherical Silica Purity  : 99.999 % Carbon Load  : 3 % Surface Area  : 340 m2/gram Pore Size  : 100 A PH Range  : 2 to 7 Particle size  : 5um and 10um

PFP Column The  Zodiac PFP column  is a high-performance chromatography solution designed for superior selectivity and retention. As a pentafluorophenyl (PFP) phase with a propyl spacer, this bonded phase delivers  reversed-phase separations  that stand apart from conventional  C18 silica surfaces . The presence of highly electronegative fluorine atoms on the periphery of the  phenyl ring ligand  allows for unique  aromatic selectivity , making it an excellent choice for challenging separations. PFP Column Exceptional Interaction Types for Enhanced Retention The  Zodiac PFP column  exhibits multiple interaction mechanisms that contribute to its high performance, including: Dispersive interactions : Effective for nonpolar compounds Dipole-dipole interactions : Enhancing selectivity for polar analytes Pi-pi interactions : Ideal for aromatic compounds Charge-transfer interactions : Providing superior retention for halogenated species These ...

C18 Column C18 columns are designed as versatile, high-performance tools for analytical applications, offering excellent resolution and retention of complex mixtures. Ideal for a wide range of compounds, these columns deliver faster results with superior peak symmetry and high performance. C18 Column Key Features of C18 Column: Spherical Silica: Provides uniform packing for reproducible results and efficient separations. High Silica Purity: 99.999% silica ensures minimal impurities for consistent, reliable performance. 24% Carbon Load: Optimized for high retention and separation across various compound types. 440 m²/gram Surface Area: Enhances resolution and interaction with analytes for better separations. Wide pH Range: Operates effectively between pH 2 and 10, suitable for diverse mobile phases. Available Particle Sizes: 3µm, 3.5µm, 5µm, and 10µm for flexibility in flow rates and separation efficiency. C18 column also features  protective end-capping , preventing peak tail...

Understanding the HPLC Test: A Comprehensive Guide to High-Performance Liquid Chromatography High-Performance Liquid Chromatography ( HPLC ) is a powerful and widely used analytical technique that plays a crucial role in separating, identifying, and quantifying compounds within complex mixtures. The HPLC test is indispensable in many scientific and industrial applications, including pharmaceuticals, food safety, environmental testing, and clinical diagnostics. In this blog, we’ll dive into the details of the HPLC test , how it works, and its importance in various industries. What is an HPLC Test? The HPLC test refers to the process of analyzing a sample using High-Performance Liquid Chromatography (HPLC) . This test utilizes a chromatographic technique to separate individual components of a mixture, allowing researchers to identify and quantify specific substances. In the test, a sample is passed through a column containing a stationary phase, and the components of the sample in...

Exploring the Different HPLC Column Types: Choosing the Right One for Your Separation Needs High-Performance Liquid Chromatography ( HPLC ) is one of the most widely used analytical techniques in laboratories around the world. HPLC allows scientists and researchers to separate, identify, and quantify components in complex mixtures, and it plays a critical role in industries ranging from pharmaceuticals to food safety. A key element in achieving precise separations in HPLC is the HPLC column —the heart of the chromatographic system. There are various HPLC column types , each designed to cater to different analytical needs. Understanding the differences between these column types is essential for selecting the right one for your specific application. In this blog, we will dive into the different HPLC column types , their uses, and how to choose the appropriate column for your chromatography needs. What is an HPLC Column? An HPLC column is a tubular device packed with a stationary ph...