HILIC Columns
HILIC Columns The Technology: HILIC (Hydrophilic Interaction Liquid Chromatography) is an advanced chromatographic method ideal for the separation and retention of polar species, including polar neutral and ionized analytes. It is a variation of normal-phase chromatography, but with key differences in the mobile phase composition and separation mechanism. While normal-phase chromatography typically employs 100% organic mobile phases, HILIC uses water-miscible organic solvents, creating a unique polar environment. Historically, HILIC phases relied on unbound silica, but recent developments have introduced bonded polar phases classified into acidic, basic, and neutral chemistries. Zodiac’s HST HILIC columns encompass these modern bonded polar phases to address a wide range of analytical challenges. HILIC Columns The Methodology: Typical Mobile Phase Composition: The mobile phase commonly includes MeCN with a small water content . Mechanism of Separation: A water-rich la...