HPLC Chromatography: Understanding HPLC, Test Methods, Instrumentation & Columns

 What Is Analytical Chemistry? Analytical chemistry is a branch of chemistry focused on the identification, separation, and quantification of matter . It plays a foundational role in scientific research, quality control, drug development, environmental monitoring, and more. Whether you're measuring the concentration of active ingredients in a pharmaceutical formulation or detecting trace contaminants in water, analytical chemistry provides the tools and methods to obtain accurate, reliable data . Core Principles of Analytical Chemistry Analytical chemistry is built upon several fundamental principles: Precision and Accuracy : The ability to measure consistently and closely to the true value. Sensitivity : The smallest quantity of a substance that can be reliably detected. Selectivity : The ability to differentiate the target analyte from other substances. Linearity : The range over which a method gives proportional responses. Limit of Detection (LOD) and Limit ...

What Are HPLC Vials? HPLC vials are small containers specifically designed for use in High-Performance Liquid Chromatography (HPLC) systems. These vials store sample solutions before they're introduced into the chromatographic system via an autosampler. Although small and often overlooked, HPLC vials are crucial for maintaining sample integrity, preventing contamination, and ensuring reproducible results. The right HPLC vial can improve sample stability, minimize loss, and prevent errors in both qualitative and quantitative analyses. Types of HPLC Vials There are several types of HPLC vials , classified based on design, closure type, and volume. Choosing the correct type depends on your sample, injection system, and application. 1. Screw Top Vials Most common type for autosamplers. Easy to use with reusable caps. Often used in general-purpose labs and QA/QC environments. 2. Crimp Top Vials Secure sealing with a crimping tool. Ideal for long-term storage or...

HILIC Columns The Technology: HILIC (Hydrophilic Interaction Liquid Chromatography) is an advanced chromatographic method ideal for the separation and retention of polar species, including polar neutral and ionized analytes. It is a variation of normal-phase chromatography, but with key differences in the mobile phase composition and separation mechanism. While normal-phase chromatography typically employs 100% organic mobile phases, HILIC uses water-miscible organic solvents, creating a unique polar environment. Historically, HILIC phases relied on unbound silica, but recent developments have introduced bonded polar phases classified into acidic, basic, and neutral chemistries. Zodiac’s HST HILIC columns encompass these modern bonded polar phases to address a wide range of analytical challenges.   HILIC Columns The Methodology: Typical Mobile Phase Composition: The mobile phase commonly includes  MeCN with a small water content . Mechanism of Separation: A  water-rich la...