HPLC Chromatography: Understanding HPLC, Test Methods, Instrumentation & Columns

What Are HPLC Vials? HPLC vials are small containers specifically designed for use in High-Performance Liquid Chromatography (HPLC) systems. These vials store sample solutions before they're introduced into the chromatographic system via an autosampler. Although small and often overlooked, HPLC vials are crucial for maintaining sample integrity, preventing contamination, and ensuring reproducible results. The right HPLC vial can improve sample stability, minimize loss, and prevent errors in both qualitative and quantitative analyses. Types of HPLC Vials There are several types of HPLC vials , classified based on design, closure type, and volume. Choosing the correct type depends on your sample, injection system, and application. 1. Screw Top Vials Most common type for autosamplers. Easy to use with reusable caps. Often used in general-purpose labs and QA/QC environments. 2. Crimp Top Vials Secure sealing with a crimping tool. Ideal for long-term storage or...

HILIC Columns The Technology: HILIC (Hydrophilic Interaction Liquid Chromatography) is an advanced chromatographic method ideal for the separation and retention of polar species, including polar neutral and ionized analytes. It is a variation of normal-phase chromatography, but with key differences in the mobile phase composition and separation mechanism. While normal-phase chromatography typically employs 100% organic mobile phases, HILIC uses water-miscible organic solvents, creating a unique polar environment. Historically, HILIC phases relied on unbound silica, but recent developments have introduced bonded polar phases classified into acidic, basic, and neutral chemistries. Zodiac’s HST HILIC columns encompass these modern bonded polar phases to address a wide range of analytical challenges.   HILIC Columns The Methodology: Typical Mobile Phase Composition: The mobile phase commonly includes  MeCN with a small water content . Mechanism of Separation: A  water-rich la...

  The column is made of spherical, porous, Polystyrene Divinyl benzene copolymer of 6.7% crosslinkage, with sulphonyl functional groups and with particle size of 9µm. Advantages: 1. Columns are packed with chemically very stable polymeric phases of PS /DVB. 2. The varied percentage cross-linkage of copolymers and varied particle sizes gives choice of selecting required column. 3. High temperature stability is exhibited by the columns (up to 95°C). 4. Excellent performance and consistency is guaranteed from column to column and batch to batch. 5. Choice of eluents can be used depending on the column and compound requirement, the simplest eluent being is Water. Technical Details    Material type  : Polymer (PS/DVB) Cross Linkage  : 6.7% Particle size  : 9 µm

C8 Column Zodiac C8 Column, An excellent resolution for critical separations, faster analysis compared to C18 increased selectivity, exceptional reproducibility. Zodiac C8 Column is an admirable choice for separations that need marginally more polar phase than C18. The high surface area that is about 440m2/g in Zodiac C8 column provides additional resolution to polar phase. Zodiac C8 column is less hydrophobic column with high surface area, optimal carbon load and unique bonded silica packing. C8 Column This column is ideal choice when analytical laboratories need extra selectivity and more speed than the C18 column. The superior resolution of Zodiac C8 yields out and out separation first time, speeding up method development and validation. These columns are highly stable and produce ever reliable results with an extended column life. The column comes with protective end-capping for a better peak without tailing and high durability of column. Faster, sharp and reproducible responses ar...

Phenyl Column Phenyl Column ü Extraordinary resolution ü Excellent choice for separating mixture of polar and non polar analytes ü Superior selectivity of structurally comparable compounds Employing ultra pure, higher stability silica and unique bonding technology, Zodiac-Phenyl columns are exceptionally designed to ensure maximum performance. Zodiac- Phenyl columns contain carbon load of about 15.0% which gives best required retention. The silica phase packing and end capping of column is completely controlled, that ensures column-to-column reproducibility. The uniform ultra pure silica particles have high surface area of 440m2/g with a controlled pore size of 100Å. Zodiac-Phenyl columns show great selectivity and peak symmetry with fairly high retention for separation of mixtures of polar and non polar compounds. The pi-pi interactions in the phenyl stationary phase influence the solute pi electrons which causes ultimate separation of compounds containing isomers. The column is conve...

Silica Column Zodiac Silica columns are made with spherical, highly pure silica particles (99.999% Pure). Zodiac Silica is the basis for our high quality Zodiac stationary Phase manufactured to provide maximum bonded phase coverage. Zodiac Silica column provides excellent peak shapes using simple eluents while operating at low pressure. Silica Column TECHNICAL DETAILS OF SILICA COLUMN Silica type  : Spherical Silica Purity  : 99.999% Carbon Load  : 0% Surface Area  : 450m2/gram Pore Size  : 100 A pH Range  : 2.0-6.0 Particle Size  : 3um, 3.5um, 5um and 10um